A pipette (sometimes spelled as pipet) is a type of laboratory tool commonly used in chemistry and biology to transport a measured volume of liquid, often as a media dispenser.The adjustable micropipette is a Wisconsin invention developed through interactions among several people, primarily inventor Warren Gilson and Henry Lardy, a professor of biochemistry at the University of Wisconsin–Madison.However, since they rely on air displacement, they are subject to inaccuracies caused by the changing environment, particularly temperature and user technique.Afterwards, the co-founder of the biotechnology company Eppendorf, Dr. Heinrich Netheler, inherited the rights and initiated the global and general use of micropipettes in labs.To minimize the possible development of musculoskeletal disorders due to repetitive pipetting, electronic pipettes commonly replace the mechanical version.These are similar to air displacement pipettes, but are less commonly used and are used to avoid contamination and for volatile or viscous substances at small volumes, such as DNA.The major difference is that the disposable tip is a microsyringe (plastic), composed of a capillary and a piston (movable inner part) which directly displaces the liquid.The safety regulations included the statement: "Never pipette by mouth KCN, NH3, strong acids, bases and mercury salts".[7][8] The Winkler–Dennis gas combustion pipette, developed by Clemens Winkler and refined by Louis Munroe Dennis, is an apparatus for the controlled reaction of liquids under a mild electric current and a supply of oxygen.At the tip of the device, a localized flow zone is created which allows for constant control of the nanolitre environment, directly in front of the pipette.[18] During repetitive tasks such as pipetting, maintaining body positions that provide a maximum of strength with the least amount of muscular stress is important to minimize the risk of injury.[20] An alternative technology, especially for transferring small volumes (micro and nano litre range) is acoustic droplet ejection.
VIAFLO electronic multichannel pipettes from INTEGRA Biosciences
Several sizes of volumetric pipette.
Pasteur pipettes with rubber bulbs attached.
A transfer pipette
Combustion pipette
A biochemist pipetting
A smart pipette stand that can control electronic pipettes