[1] During this process, the constant-region portion of the antibody heavy chain is changed, but the variable region of the heavy chain stays the same (the terms variable and constant refer to changes or lack thereof between antibodies that target different epitopes).[2] Naïve mature B cells produce both IgM and IgD, which are the first two heavy chain segments in the immunoglobulin locus.This allows AP-endonucleases to cut the newly-formed abasic site, creating the initial SSBs that spontaneously form DSBs.[7] The intervening DNA between the S-regions is subsequently deleted from the chromosome, removing unwanted μ or δ heavy chain constant region exons and allowing substitution of a γ, α or ε constant region gene segment.[9] With the exception of the μ and δ genes, only one antibody class is expressed by a B cell at any point in time.