Luxol fast blue stain

[1] LFB is commonly used to detect demyelination in the central nervous system (CNS), but cannot discern myelination in the peripheral nervous system.[2] Luxol fast blue is a copper phthalocyanine dye that is soluble in alcohol and is attracted to bases found in the lipoproteins of the myelin sheath.Tissues sections are treated over an extended period of time (usually overnight) and then differentiated with a lithium carbonate solution.[2] It is often combined with H&E stain (hematoxylin and eosin), which is abbreviated H-E-LFB, H&E-LFB.Other common staining methods include the periodic acid-Schiff, Oil Red O, phosphotungstic acid, and Holmes silver nitrate method.
Micrograph of the pons using a hematoxylin & eosin -luxol fast blue stain.
Coronal section of a mouse brain stained with Hematoxylin & LFB
Micrographhematoxylin & eosinmyelinlight microscopyHeinrich Klüverdemyelinationcentral nervous systemperipheral nervous systemcopper phthalocyaninelipoproteinsmyelin sheathneuropilnerve cellslithium carbonateH&E stainhematoxylinperiodic acid-SchiffOil Red OBielschowsky stainMicrobial and histological stainshemosiderinPerls Prussian blueLipidsSudan stainSudan IISudan IIISudan IVSudan Black BCarbohydratesAlcian blueMucicarminePeriodic acid–Schiff stainAmyloidCongo redThioflavinBacteriaGram stainMethyl violetGentian violetSafraninAcid-fastZiehl–Neelsen stainKinyoun stainCarbol fuchsinFuchsineMethylene blueAuramine–rhodamine stainAuramine ORhodamine BAuramine phenol stainConnective tissuetrichrome stainMasson's trichrome stainLillie's trichromeLight Green SF yellowishBiebrich scarletPhosphomolybdic acidFast Green FCFSirius RedVan Gieson's stainCresyl violetCyanineJaswant Singh–Bhattacharji (JSB) stainHaematoxylinEosin YJanus Green BGiemsa stainGömöri trichrome stainMethyl blueMoeller stainMovat's stainNeutral redSchaeffer–Fulton stainSilver stainGrocott's methenamine silver stainWarthin–Starry stainWright's stainAcidophilicBasophilicChromophobic