Raised again in 1939 for service during World War II, the brigade was deployed to the Middle East in early 1940 and subsequently saw action in the Western Desert and in Greece in 1941.In 1942, it returned to Australia in response to Japan's entry into the war, and later the brigade played a prominent role in the Kokoda Track campaign and at Buna–Gona in Papua.Withdrawn to Australia in early 1943, the 16th Brigade was re-organised and received many replacements from disbanding formations, but it was not recommitted to combat operations until late in the war.[1] During World War I, the brigade was briefly re-formed as part of the all volunteer First Australian Imperial Force (AIF).Consisting of four infantry battalions – the 2/1st, 2/2nd, 2/3rd and 2/4th – the brigade was the first raised as part of the all volunteer Second Australian Imperial Force (2nd AIF).[7] In January 1941, the brigade was committed to the Western Desert Campaign, going into action for the first time around Bardia, which was captured from the Italians over several days.A German breakthrough at Tempe Gorge resulted in a further withdrawal during which the 2/2nd and 2/3rd Battalions provided rearguard security as Anzac Corps withdrew through Larissa.In early 1942, following Japan's entry into the war, the Australian government requested that the 6th Division be returned to Australia and in February the brigade moved to Beit Jirja, in Palestine, prior to embarkation.Heavily depleted by disease and casualties, by December 1942, the 16th Brigade was withdrawn by air back to Port Moresby before returning to Australia in January 1943.In December 1944, the brigade was committed to the Aitape–Wewak campaign in New Guinea, when the Australians took over from US forces, which had established a base at Aitape, where they joined the rest of the 6th Division in an advance towards Wewak and then into the Torricelli Range.
Troops from the 2/1st Battalion at Wondecla, Queensland, April 1944